Structure and function of SirC from Bacillus megaterium: a metal-binding precorrin-2 dehydrogenase
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منابع مشابه
Structure and function of SirC from Bacillus megaterium: a metal-binding precorrin-2 dehydrogenase.
In Bacillus megaterium, the synthesis of vitamin B(12) (cobalamin) and sirohaem diverges at sirohydrochlorin along the branched modified tetrapyrrole biosynthetic pathway. This key intermediate is made by the action of SirC, a precorrin-2 dehydrogenase that requires NAD(+) as a cofactor. The structure of SirC has now been solved by X-ray crystallography to 2.8 A (1 A = 0.1 nm) resolution. The p...
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One of the four operons required for cobalamin biosynthesis in Bacillus megaterium is also associated with sirohaem synthesis, and contains three genes, sirA, sirB and sirC. By undertaking functional complementation experiments and in nitro assays using recombinantly produced enzymes, we have been able to demonstrate that (1) SirA acts as a uroporphyrinogen I I1 methyltransferase, transforming ...
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Received for publication, April 9, 1960. higher organisms. The bacterial cytoplasm consists of granules, about 20 m/~ in diameter, which contain numerous enzymes of the electron transport system of the cell (1) and which were shown to carry out oxidative phosphorylation (2). Attempts of Mudd et al. (3) and others to visualize mitochondria-like bodies by means of intravital reduction of tetrazol...
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No. ponn1490 Beamline(s): X12C Cells of Bacillus and Clostridium species produce spores when one or more nutrients are limiting for growth of these organisms. The spores DNA is saturated with a group of small, acid-soluble proteins (SASP) of the α/βtype. In spore germination SASP are degraded to amino acids and this degradation is initiated by a sequence specific protease called the germination...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 2008
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj20080785